Abstract: The review considers the experiments of biologicalmechanics with coupling of force and electric field with biological moleculesand materials in probe techniques. Experimental methods and mainresearch findings are summarized systemically about the mechanicalproperties of biological molecules and materials at nano-scale at the tipsof scanning probe microscope, optical tweezers, magnetic tweezers and so on.Then the advances in molecular biological and physical mechanics studies inthe tiny and super-special environment between the tip and the substrateare discussed. Probe techniques have significant importance in revealing thephysical mechanical properties of biological molecules (including DNA andproteins), characterizing the structures of biological materials and formolecular bionics. It is envisaged that molecular biological and physicalmechanics studies in the multi-field coupling environments under the probetips will be an important field for future researches. It is necessary tocombine experimental investigations with theoretical analyses andcomputational simulations in the tip mechanics, and the development inthe virtual experimental technique of molecular physical mechanics will be animportant trend.